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Saturation Transfer Difference experiments reveal that two pamoic acid protons are essential in the binding to the 8 kDa domain. a. Superposition of STD spectra, without (in blue) and with (in red) selective saturation of the 8 kDa domain protons. Only Hd and He pamoic acid protons revealed binding to the 8 kDa domain. b. Plot showing STD amplification factor of He versus pamoic acid concentration. Fitted curve is represented in red, corresponding to Kd value of 13 μM ± 5 μM.
