Figure 7.

Comparison of qRT-PCR and microarray expression data for AP2-EREBP genes. The five AP2-EREBP genes found up-regulated in the microarray data (see Figure 6) and two AP2-EREBP genes that were not significantly differentially expressed as detected on the microarray were tested for RNA abundance using quantitative real time RT-PCR. As an internal standard, expression levels of At5g62700 (TUB, tubulin) was used to determine relative expression levels in each sample (ΔCT values). Shown in green are the ΔΔCT values comparing ΔCT values of DBM treated samples with untreated control samples at 1 h, 4 h, 8 h, 12 h, and 24 h of continuous feeding (right scale bar). For comparison, normalized log2-expression ratios found using the microarray platform are given in blue (left scale).