Figure 2.

Resistance of FANCA-mutant cells with defective FANCD2 function to hydrogen peroxide (H₂O₂). (a) Representative images illustrating staining for subnuclear FANCD2 foci in isogenic fibroblast pairs, either deficient for FANCD2 (PD20) or FANCA (PD220) and their respective wild-type (wt) complemented counterparts. Foci were visualized three hours after treatment with H₂O₂ (25 μM for 2 hours). (b) Clonogenic survival of cells with or without wild-type FANCA after treatment with varying concentrations of H₂O₂. Data represent logarithmic means +/− standard error from 15-repeat experiments.