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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Mol Cell Neurosci. 2007 Sep 14;37(1):170–177. doi: 10.1016/j.mcn.2007.09.004

Figure 1. The genomic organization of the mouse CCR2 gene.

Figure 1

A) Reported mRNA species and their coverage on genomic DNA sequences (numbered boxes: exons; lines between exons: introns; a black box: protein coding region). B) Primer extension experiment. The location of the primer is denoted in the diagram on the left (W: WEHI265.1 monocytic cell line). C) RT-PCR experiments. Relative location of primers is denoted on the upper diagram (arrow heads: primers). Two sets of primers were used: E1up/E3dn and E2up/E3dn (left two and right two lanes, respectively). The structure of the PCR product is denoted in the left and right diagrams (W: WEHI265.1 monocytic cell line; F: F11 DRG neuronal cell line). D) The sequence of the exon 1 (E1). Exonic sequence is in upper cases and non-exonic sequence in lower cases. An additional intronic sequence within the exon 1 is underlined. AG and GT consensus splice dinucleotides are in bold letters. An mRNA with this sequence spliced out corresponds to the shorter band in the leftmost lane in C.