Figure 3. The mcl1-101 and swi7-H4 mutants display alleviation of transcriptional gene silencing at both central core and outer repeat regions.

(A) (upper panel) The schematic diagram of ura4 ⁺ and ade6 ⁺ genes inserted into imr1L and otr1R, respectively. Note that the ura4 ⁺ gene is inserted into heterochromatic domain, which is defined as outside of tRNA genes (vertical lines) [23]. (lower panel) Tenfold serial dilutions were spotted. (B) (upper panel) The schematic diagram of the ura4 ⁺ gene inserted into cnt1. (lower panel) Tenfold serial dilutions were spotted.