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. 2008 May 21;3(5):e2221. doi: 10.1371/journal.pone.0002221

Figure 5. Acetylation of histone H4 is aberrantly increased in the central kinetochore domain in mcl1 and swi7 mutants.

Figure 5

(A) ChIP-on-chip was performed using the antiserum raised to a peptide corresponding to amino acid 2–19 of histone H4 that is acetylated at K5, K8, K12, and K16 (AcH4-KN). The cells were prepared as described in Figure 4C. Values obtained from AcH4-KN in the mcl1 mutant were divided by those in wild-type strain. The orange shading represents the binding ratio of loci that show significant enrichment as described previously [68]. Representative results for subtelomeric and centromeric regions of chromosome I are shown. (B,C) ChIP was performed using AcH4-KN (B) and AcH4-K16 (C), respectively. The cells were prepared as described in Figure 4C. Values obtained in the mutants were normalized to those obtained in wild-type strain. Values are further normalized to euchromatic lys1 locus. Average fold enrichment compared to wild-type are obtained from two repetitions. Strains were wild-type (JY879), mcl1-101 (NYSPC52), swi7-H4 (TN403).