Figure 6. Multi-copy suppression of mcl1-101 phenotypes by sir2 ⁺ and clr3 ⁺ HDAC genes.

Wild-type and mcl1-101 mutant harboring the ura4 ⁺ insertion at the cnt1 were transformed with multi-copy plasmid carrying sir2 ⁺, clr3 ⁺, or both, respectively. Tenfold serial dilutions were spotted onto EMM-Leu and EMM-Leu containing 5-FOA (A) or EMM-Leu containing indicated concentration of TBZ (B). In panel A, sir2 ⁺ and, to a lesser extent, clr3 ⁺ partially suppressed 5-FOA sensitivity of the mcl1-101 mutant. In panel B, clr3 ⁺ partially suppressed TBZ sensitivity of the mcl1-101 mutant, and sir2 ⁺ also relieved the sensitivity only in the presence of clr3 ⁺.