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. 2002 Jul 2;87(2):181–186. doi: 10.1038/sj.bjc.6600419

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Copyright © 2002 Cancer Research UK

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(A) 28 S and 18 S rRNA bands. Total RNA extraction as visualised in ethidium bromide stained 2% agarose gel. (B) Ethidium bromide-stained gel visualisation of RT–PCR products showing a 207 bp band in the positive samples. Lane 1. Molecular Weight Marker (ΦX174 RF DNA HaeIII Digest). Lanes 2–4. Positive samples (three melanoma patients). Lanes 5–7. Negative samples (three melanoma patients). Lane 8. Positive control (SK-mel 28 cell line). Lane 9. Negative control (healthy donor). Lane 10. Negative control (sample without cDNA).