Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Aug 12;87(4):423–431. doi: 10.1038/sj.bjc.6600456

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Alignment of the M41 gene and M41 transcripts. The gene region of chromosome 21q22.1 is shown at the top with the exons of the DS-CAM gene shown as vertical bars. The location of the M41 gene is shown as a square to scale beneath the genomic DNA and expanded beneath. The location of response elements for oestrogen (ERE) and progesterone (PRE) and Alu sequences are shown. The region of the original M41 cDNA isolated from the MCF-7 subtracted library, and processing variants of M41 mRNA (A1–A7) obtained from the RACE reactions are shown as horizontal bars with gaps indicating the intronic regions. Numbering refers to the base number of contig NT_011512.3. The 5′ intron exon boundaries revealed by the RACE reactions at 27328651, 27328698, 27328827, 27329381, and the 3′ intron exon boundaries at 27328749, 27328894, 27329821 (enumeration of contig NT_011512.3) all contained conserved GT / AG sequences and exhibited 62.5, 75, 87.5, 100, 75, 93.75, and 81.25% identity, respectively, to the consensus sequences for 5′ or 3′ intron exon boundaries (Mount, 1982). The symbols (A) at the ends of the lines indicate the poly(A) addition sites. Variants contained one of two alternative poly(A) addition sites (at 27330601 and 27330228 of NT_011512.3), with a consensus AATAAA poly(A)-addition signal, 29 and 19 nucleotides upstream of the poly(A)-addition sites, respectively. The lengths of the proposed mRNA variants arising from the 7 alternatively-spliced exons correspond broadly to the sizes of two of the bands observed in the Northern blot for the mammary tumour cell line MCF-7 (Figure 1), strongly suggesting that the RACE products are defining near full length mRNAs. Horizontal arrows indicate the direction of transcription of the genes.