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. 2002 Oct 7;87(8):859–867. doi: 10.1038/sj.bjc.6600545

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Copyright © 2002 Cancer Research UK

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Cell adhesion to LAP is αvβ6-dependent. Chromium [⁵¹Cr]- labelled cells (1.5×10⁴) were added to LAP-coated 96-well plates containing an irrelevant control antibody (W632 anti-MHC class 1) or test antibodies against αv (L230), αvβ5 (P1F6), αvβ6 (10D5), β1 (P4C10) and α5β1 (P1D6). Background binding to BSA has been subtracted from the results. Figures show representative experiments performed in quadruplicate. Error bars represent standard deviation. (A) VB6 cells show increased adherence LAP. β6 transfected cells (VB6), control cells (C1) and αv-negative cells (H357) were plated onto varying concentrations of LAP. VB6 cells showed significantly increased adhesion compared to C1 cells (which express low levels of endogenous αvβ6). H357 cells did not adhere. (B) Adhesion to LAP is αvβ6-dependent. VB6 and C1 adhesion to LAP was inhibited by anti-αvβ6 antibody or anti-αv antibody. Antibodies against αvβ5, α5β1 or β1 produced no effect. These data suggest that adhesion of VB6 and C1 cells is modulated solely through αvβ6.