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. 2002 Oct 7;87(8):909–917. doi: 10.1038/sj.bjc.6600541

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Copyright © 2002 Cancer Research UK

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Staurosporine induced a time dependent increase in annexin-V binding in (A) T47D and (B) MCF-7 cells. Both T47D and MCF-7 cells were treated with 1 μM STS for the times indicated. Cells were washed in PBS and stained with annexin-V-FITC and PI and analysed by Flow Cytometry. Cells staining annexin-V-FITC+/PI- were considered apoptotic; cells staining annexin-V-FITC+/PI+ were classified as late apoptotic/necrotic. Data are presented as per cent of cells in each classification. Error bars represent the mean±s.e.m. of three independent experiments.