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. 2002 Nov 4;87(10):1119–1127. doi: 10.1038/sj.bjc.6600614

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Copyright © 2002 Cancer Research UK

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Factor H/FHL-1 binding to ovarian tumour cells. (A) Binding of ¹²⁵I-labelled factor H and FHL-1 to ovarian tumour cells SW626, PA-1, SK-OV-3 and Caov-3. Cells (93×10⁵) were incubated with radiolabelled factor H (30 ng, 6.6×10⁶ c.p.m. μg⁻¹) or FHL-1 (30 ng, 3.3×10⁶ c.p.m. μg⁻¹) for 1 h at 37°C in 1 : 3 GVBS, pH 7.4. The results (mean±s.d.; n=3) are expressed as the amount of bound protein (ng). A representative of three experiments with similar results is shown. (B) Dose-response analysis of binding of ¹²⁵I-labelled factor H and FHL-1 to SK-OV-3 cells. (C) Inhibition analysis of binding of ¹²⁵I-labelled factor H to SK-OV-3 cells. Indicated amounts of unlabelled factor H or BSA (up to a 100-fold excess) were incubated together with ¹²⁵I-labelled factor H as in (A).