Figure 3.

(A) IGF-I-induced tyrosine phosphorylation. Analysis of IGF-I-induced tyrosine phosphorylation in Leishmania (L.) mexicana promastigotes (lanes a–d) and amastigote-like forms (lanes e–h) was done by immunoblotting with a monoclonal antiphosphotyrosine antibody. Stimulation time: 0, lanes a and e; 1 min, lanes b and f; 5 min, lanes c and g; and 25 min, lanes d and h. (B) Quantitative analysis of the IGF-I-induced tyrosine phosphorylation in Leishmania (L.) mexicana amastigote-like forms. Evaluation of the phosphorylation in Leishmania (L) mexicana promastigotes at 0 (broken line) and 5 min (solid line) of IGF-I induction was performed by using laser densitometry.