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. Author manuscript; available in PMC: 2009 May 2.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 27;369(2):781–787. doi: 10.1016/j.bbrc.2008.02.093

Fig. 2. Knockdown of Rictor blocks MHC class I-induced phosphorylation of ERK at Thr202/Tyr204.

Fig. 2

A, EC were transfected with 100 nM Rictor siRNA or GL-2 control siRNA. After 48 h the EC were stimulated with 1 μg/mL anti-MHC class I mAb W6/32 for various time points. EC were lysed and immunoblotted with antibodies to Rictor, Vinculin, ERK Thr202/Tyr204 and ERK. B, EC were transfected with 100 nM Raptor siRNA or GL-2 control siRNA. After 48 h the EC were stimulated with anti-MHC class I mA at various time points. EC were lysed and immunoblotted with antibodies to Raptor, Vinculin, ERK Thr202/Tyr204 and ERK. Densitometry results are expressed as the percentage maximal increase in phosphorylation above control values (mean ±S.E.). Data is representative of three independent experiments.