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. Author manuscript; available in PMC: 2009 May 2.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Feb 27;369(2):781–787. doi: 10.1016/j.bbrc.2008.02.093

Fig. 3. Integrin stimulates ERK phosphorylation through Rictor.

Fig. 3

A, EC were transfected with 100 nM Rictor siRNA or GL-2 control siRNA. After 48 h EC were trypsinized, kept in suspension (Suspend) for 30 min and then replated on poly-L-lysine (Lys) or fibronectin (FN) coated dishes for 10 min. EC were lysed and immunoblotted with antibody to ERK Thr202/Tyr204, ERK and Rictor. Data is representative of three independent experiments. B, EC were transfected with 100 nM Rictor siRNA or GL-2 control siRNA for 48 h. EC were stimulated with 1 ng/mL or 20 ng/mL bFGF for 10 or 30 min. EC were lysed and immunoblotted with antibodies to ERK Thr202/Tyr204, ERK, Rictor and Vinculin. Data is representative of three independent experiments.