FIGURE 3.

Effect of AMAP on liver GSH levels and JNK activation. A, total liver homogenate GSH; B, mitochondrial GSH; C, JNK activation. C57BL/6 mice were treated either APAP (400 mg/kg) or AMAP (600 mg/kg) dissolved in warm PBS. 2 h following APAP or AMAP treatment, liver mitochondria was isolated. Total liver homogenate and mitochondria GSH levels were measured using HPLC with electrochemical detection, and Western blot analysis was performed using antisera against phospho-JNK, JNK, and actin as loading control. n = 4 mice.