FIGURE 7.

Time course of JNK translocation to mitochondria following APAP treatment in vivo: modulation by JNK inhibitor. A, time course of JNK translocation to mitochondria following APAP treatment. B, JNK inhibitor prevents JNK translocation to mitochondria (4 h following APAP treatment). C57BL/6 mice were treated APAP (600 mg/kg) dissolved in warm PBS and pretreated with either JNK inhibitor (10 mg/kg in DMSO (8.3%) and PBS) or equivalent amounts of DMSO in PBS 1 h prior APAP treatment. At indicated times livers were taken, and mitochondria were separated from cytoplasm by differential centrifugation. Western blot analysis was performed using antisera against phospho-JNK, JNK, and cytochrome oxidase as loading control.