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. 2008 May 16;283(20):13850–13855. doi: 10.1074/jbc.M709118200

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Copyright © 2008, The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Cellular organization of enolase. Immunofluorescence micrographs using monoclonal anti-HA antibody. The chromosomal copy of eno is tagged with the HA epitope tag. A, strain AT29 (rne^1–417 eno::HA). B, AT60 (rne^1–417 ΔrhlB eno::HA). C, AT30 (rne^1–659 eno::HA). D, AT63 (Δpnp,ΔrhlB eno::HA). E, strain AT58 (ΔrhlB eno::HA). Where rne was replaced by rne^1–417 the strains grew as mixed populations of filaments and short cells (5). The described localization pattern was present in 95–98% of cells (see “Experimental Procedures”). Scale bar, 1 μm.