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UV-visible absorption spectra of reconstituted A. vinelandii NfuA as prepared (solid line) and after anaerobic reduction with one reducing equivalent of dithionite (dashed line). Molar extinction coefficients are expressed per NfuA monomer. The inset shows the X-band EPR spectrum of the sample reduced with one reducing equivalent of dithionite. EPR conditions: microwave frequency, 9.60 GHz; microwave power, 10 milliwatt; modulation amplitude, 0.63 mT; temperature, 10 K.
