FIGURE 3.

Mössbauer spectrum of ⁵⁷Fe-reconstituted A. vinelandii NfuA recorded at 4.2 K with a field of 50 mT applied parallel to the γ-radiation. The solid black line is the composite spectrum including the simulated spectrum of a [4Fe-4S]²⁺ cluster (dashed line), scaled to 90% of the total iron absorption, and the simulated spectrum of a Fe²⁺ species (dotted line), accounting for 10% of the iron absorption. The parameters used for the simulations are provided in the text.