FIGURE 7.

Competition of BMP/GDF propeptides for rF23. Immobilized BMP/GDF propeptides were injected with 20 nm rF23 in the presence of increasing concentrations of different inhibitor propeptides. The signal in resonance units (RU) obtained for rF23 at 20 nm without competitor was set as 100%. The decrease of the 100% rF23 signal was graphed against the inhibitor concentrations to determine the inhibition constant for each competition reaction (Table 4). To account for variations of the rF23 signal due to buffer changes caused by the addition of different amounts of competitor, we generated for each competition sensorgram a buffer-matched control without competitor, which was set in each case as the 100% reference signal.