FIGURE 5.

Effect of RCAN1 siRNA and activated ATF6 on ANP and BNP gene induction by phenylephrine. NRVMC were transfected with either control siRNA (Con siRNA) or RCAN1 siRNA, and then infected with AdV-Con or AdV-ATF6. 24 h later, cultures were treated ± phenylephrine (50 μm) for 24 h. Cultures were then extracted and ANP (panel A), or BNP (panel B) mRNA levels were determined by RT-qPCR. Shown are mean values of each target gene/GAPDH mRNA, expressed as the -fold of control (bar 1) ± S.E. for each treatment (n = 3 cultures per treatment). ^* and §, p ≤ 0.05 different from all other values.