TABLE 1.

Comparison of specificity triad residues

Peptidase	Specificity triad	P1 preference	Source/Reference
Guinea pig chymase	SAV	Leu >> Met > Phe	This work
Guinea pig chymase A216G	SGV	Phe, Tyr >> Leu > Met	This work
Human chymase	SGA	Phe, Tyr >> Leu, Trp > Met	Ref. 43 and this work
Dog chymase	SGA	Phe, Tyr > Trp	Refs. 12, 22, 44, and 45
Mouse chymase 4	SGA	Phe > Tyr > Trp	Refs. 21 and 35
Rat chymase 1	SGA	Phe > Trp > Tyr >> Leu? > Met	Refs. 35, 46, and 47
Hamster chymase 1	SGA	Phe, Tyr > Leu >> Trp, His	Ref. 23
Human Pancreatic Ela 2	SGS	Met > Leu > Tyr > Phe > Ala	Ref. 48
Cattle chymotrypsin	SGG	Tyr > Phe > Trp >> Met > Leu	Ref. 49
Human PSA	SGS	Met > Ala > Tyr > Arg > Lys > Leu > Phe	Ref. 50
Rat chymase 2	AGA	Phe > Tyr >> Met	Ref. 46
Human SCCE	NGG	Tyr > Ala = Met > Phe = Leu, Arg, Lys	Ref. 50
Human granzyme H	TGG	Phe > Tyr > Met > Leu > Ala > Arg	Ref. 51
Human cathepsin G	AGE	Lys = Phe > Arg = Leu > Met > Ala > Asp	Refs. 17 and 44
Cattle duodenase	NGD	Arg, Lys, Phe, Leu, Tyr	Ref. 52
Rat chymase 4	LGI	Phe > Tyr	Ref. 26
Rat granzyme M	AST	Met > Leu	Ref. 53
Human granzyme M	ASP	Met > Leu	Ref. 54
Human neutrophil elastase	GVD	Ala > Val > Ile > Thr > Met	Ref. 49
Mouse MCP-5	NVA	Val > Ile > Ala	Ref. 19
Rat MCP-5	NVA	Val, Ala, Ile	Refs. 19 and 20
Hamster chymase 2	NVS	Ala > Val	Ref. 23
Rat granzyme B	AGR	Asp	Ref. 49
Human β-tryptase	DGG	Arg, Lys	Ref. 55
Rat trypsin	DGG	Arg > Lys	Ref. 49
Human plasmin	DGG	Lys > Arg	Ref. 49
Human thrombin	DGG	Arg > Lys	Ref. 49
Mouse MCP-2	SYA	Inactive	Ref. 36
Mouse MCP-8	ARR	Inactive	Ref. 37