Table 3.
Changes in expression of a few select ECM genes in response to some treatments that modify aqueous outflow facility. Approximate fold changes are shown from microarray, quantitative RT-PCR, or proteomics analyses. NS indicates that changes were not statistically or biologically significant, N indicates information not available, and S indicates alternative mRNA splicing has been reported. Values are at the times of maximum change and range from 12 to 72 hrs for stretch, cytokines or TGFβ and up to 10 days for dex.
| ECM Component | Stretch | TNFα | IL-1α | TGFβ | Dex |
|---|---|---|---|---|---|
| Versican | 0.45, S | 0.47, S | 0.46, S | 4.8–9, S | 0.034 |
| Tenascin C | 2.6, S | 4.5, S | 3.7, S | N | N |
| Fibronectin | 1.5, S | NS, S | NS, S | 3–4, S | 2, S |
| CD44 | 1.8, S | 1.7, S | 1.36, NS, S | N | N |
| Syndecan 1 | 0.77, NS | 0.33 | 0.23 | N | N |
| Syndecan 2 | 1.5 | 0.32 | 0.46 | N | N |
| Synedcan 4 | NS | 2.1 | 1.87 | N | N |
| Glypican 3 | N | 1.98 | 2.78 | N | N |
| SPARC | 2.4 | 0.21 | 0.48 | N | N |
| Testican 1 | N | 0.37 | 0.48 | N | N |
| Testican 3 | N | 1.79 | NS | N | N |
| Thrombospondin 1 | NS | 0.32 | 0.49 | 2.4–5 | N |
| Thrombospondin 2 | 0.68, NS | 0.12 | 0.29 | N | N |
| TSG-6 | N | 10.24 | 24.5 | 3.3 | N |
| VCAM-1 | 1.69 | 47 | 28 | 0.31 | N |
| Collagen XIV | 3.25 | NS | NS | 0.38 | N |
| Periostin | 0.49–2.2 | 4.63 | 4.93 | 5.3 | N |
| Matrix Gla protein | 2.5–7 | 0.29 | 0.31 | N | N |
| Link protein | 1.3, NS | NS | 1.34, NS | 2.1 | N |
| MMP-2 | 1.4 | NS | NS | 4.9 | N |
| MMP-3 | NS | 49.5 | 54.6 | 8 | N |
| ADAMTS 4 | N | 25.7 | 36.6 | N | N |
| ADAMTS 5 | NS | 8.3 | 18.3 | 4.0 | N |