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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: J Biochem. 2006 Mar;139(3):363–371. doi: 10.1093/jb/mvj037

Figure 2. Effects of cysteine protease inhibitors on the maturation of wild-type and D231N mutated cath-D in transfected fibroblasts.

Figure 2

(a) Effects of E-64 in fibroblasts. CD55−/−cath-D and CD55−/−D231Ncath-D cells were either untreated or treated for 48 h with 10 mM E-64 and cellular cath-D was analysed by immunoblotting.

(b) Concentration dependency of the E-64 effects. CD55−/−cath-D cells were either untreated or treated for 48 h with increasing concentrations of E-64 and cellular cath-D was analysed by immunoblotting.

(c) Effects of E-64 using two-dimensional electrophoresis. CD55−/− cath-D cells were either untreated or treated for 48 h with 10 mM E-64 and cellular cath-D was analysed by two-dimensional electrophoresis and immunoblotting.

(d) Effects of CA-074Met. CD55−/−cath-D and CD55−/−D231Ncath-D cells were untreated or treated for 48 h with 50 μM CA-074Met and cellular cath-D was analysed by immunoblotting. The effect of 10 mM E-64 is shown.

(e) Effects of CLICK-148 alone or in combination with CA-074Met. CD55−/−cath-D and CD55−/−D231Ncath-D cells were either untreated or treated for 48 h with 5 or 20 μM CLICK-148 alone or in combination with 50 μM CA-074Met. Cellular cath-D was analysed by immunoblotting.

Brackets indicate the 52-48 kDa processing intermediates detected in CD55−/−cath-D cells and the 51-47 kDa processing intermediates detected in CD55−/−D231Ncath-D cells.