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. 2007 Aug 25;63(Pt 9):761–764. doi: 10.1107/S1744309107038651

Table 2. Purification of the overexpressed human mitPheRS from 18 g E. coli Rosetta + pET-21_mitPheRS.

  Protein (mg) Total activity† (U min−1) Specific activity† (U min−1 mg−1) Purification factor Yield (%)
Crude extract 892 6000 6.7   100
DEAE Sepharose 68 4500 66 9.8 75
HiTrap heparin 12 2180 180 27 36
Superdex 200 10 2000 200 30 33
†

One unit of mitPheRS activity is defined as the amount of enzyme necessary to catalyze the attachment of 1 nmol L-[3H]-phenylalanine to the tRNA in 1 min at 310 K.