Table 1. Purification steps of RPTP.
Procedure | Volume (ml) | Protein (mg) | Total activity (U) | Specific activity (U mg−1) | Purification | Yield (%) | |
---|---|---|---|---|---|---|---|
1 | Homogenate | 9980 | 68862 | 1297422 | 18.84 | 1 | 100 |
2 | PEG + (NH4)2SO4 | 5400 | 11502 | 1293635 | 112.47 | 6 | 99.7 |
3 | Phenyl-Sepharose | 383 | 498 | 845825 | 1698.78 | 90 | 65.2 |
4 | DEAE-Toyopearl | 99.5 | 158 | 581665 | 3676.65 | 195 | 44.8 |
5 | Superdex 200 | 42 | 67 | 438626 | 6568.22 | 350 | 33.8 |