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. 2003 Mar 4;88(5):796–802. doi: 10.1038/sj.bjc.6600811

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Copyright © 2003 Cancer Research UK

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Expression of NRP-1 and EGF-R by seven human gastric cancer cell lines. Cells were grown to 80% confluence in 10% serum-containing medium. RT–PCR with NRP-1-specific primers was performed to detect NRP-1 RNA expression. Western blots were performed to detect EGF-R protein expression. NRP-1 expression closely mirrored EGF-R expression.