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. 2003 Mar 4;88(5):796–802. doi: 10.1038/sj.bjc.6600811

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Copyright © 2003 Cancer Research UK

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Effect of EGF on Erk1/2, Akt, and P38 phosphorylation in NCI-N87 cells. The cells were incubated in 5% serum-containing medium overnight and then were incubated with 50 ng ml⁻¹ EGF for the indicated duration in 1% serum-containing medium. Phosphorylated and total protein levels were determined by Western blot analyses. EGF led to induction of phosphorylated Erk1/2, Akt, and P38.