Table 1. Flow cytometric analysis of ovalbumin expression and immune cell infiltration in EL-4, progressive E.G7-OVA and regressive E.G7-OVA tumours.

	Tumour type
Antigen	EL-4	E.G7-OVA (regressive)	E.G7-OVA (progressive)
Ovalbumin	3.4±0.2	1608±480a	309±66
CD8	150±23	272±27a	174±19
CD68	64±17	177±35b	99±29

At 13 days after implantation, single-cell suspensions were prepared by disaggregating solid tumour material. These cell suspensions were then incubated with the relevant primary antibody and fluorochrome-conjugated secondary antibody, as described in the Materials and Methods section. Three tumours were used in each group and the numbers represent the mean±s.e.m. of the fluorescence intensities in arbitrary units.

^aStatistically significantly different from EL-4 and progressive E.G7-OVA tumours, P<0.01;

^bstatistically significantly different from EL-4 tumours, P<0.01.