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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2007 Aug 30;27(11):2484–2490. doi: 10.1161/ATVBAHA.107.151100

Figure 3.

Figure 3

Partial overlap of binding of coagulation factors and annexin A5 to platelets in thrombi. Human thrombi were generated on collagen under flow and coagulant conditions (see Figure 1) in the presence of AF647-annexin A5 (red) plus either 20 nmol/L AF488-factor Va, 200 nmol/L OG-prothrombin, 100 nmol/L OG-factor Xa, or 1.25 μg/mL FITC-α-CD62 mAb (green). Histograms give pattern overlap analysis of TPLSM images for annexin A5 (red) and indicated factor or antibody (green); shown is the Pearson correlation coefficient (Rr) of corresponding green and red bit maps (n = 4 to 7).