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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: Methods Mol Biol. 2005;309:273–282. doi: 10.1385/1-59259-935-4:273

Fig. 3. Circumventing crossreacting dsRNA triggers.

Fig. 3

(A) An alignment of the nucleotide sequences of exons 4.1 and 4.8. The region likely responsible for the cross-reaction is shaded in gray. (B) Smaller dsRNA triggers result in isoform-specific mRNA degradation. Small dsRNAs corresponding to the first 79 nt of exon 4.1 and 4.8 were synthesized and used to treat Drosophila cells. The abundance of each Dscam isoform was analyzed as described in Fig. 2 and the results for exon 4.1 and 4.8 are shown graphically.