Figure 3.

α-TOS sensitises Jurkat cells to TRAIL killing. Jurkat cells (0.5×10⁶ ml⁻¹) were pretreated with α-TOH, α-TOS, MG132 or hydrogen peroxide at the concentrations indicated (μM) for 4 h, or to TNF-α at 100 U ml⁻¹ for 1 h, after which they were exposed to rhTRAIL at 40 ng ml⁻¹. At time points indicated (A) or at 6 h (B) following TRAIL addition, cells were evaluated for apoptosis.