Figure 4.

α-TOS abolishes transient activation of NF-κB by TRAIL. Jurkat cells (0.5×10⁶ ml⁻¹, 10⁷ total) were treated as specified in the legend to Figure 3 (concentrations in μM except U ml⁻¹ for TNF-α, rhTRAIL at 40 ng 1⁻¹). At a 2-h time point (A) or as specified (B), cells were washed with PBS, spun down, the pellet resuspended in the lysis buffer, and the lysate probed for NF-κB activation using the TRANS-AM kit as detailed in Materials and Methods. The level of NF-κB activation (p65 binding to its cognate DNA sequence) is expressed as a relative absorbance at 450 nm.