Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2003 Jan 28;88(1):109–114. doi: 10.1038/sj.bjc.6600737

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2003 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(A) Methylation-specific PCR of CpG island of the RB1 promoter in glioblastomas (P12 and P32) and anaplastic astrocytoma (P37). Cases P62 and P64 (glioblastomas) showed only unmethylated alleles. Positive control for methylated DNA: normal DNA from lymphocytes treated with SssI methyltransferase; normal control: DNA from a non-neoplastic brain tissue. Negative control from untreated lymphocytes DNA is not shown (L: molecular weight marker). (B) Sequence (reverse of the coding strand) analysis of bisulphite-modified DNA from tumour P12 (MR) and from non-neoplastic brain tissue (UR). Tumour DNA shows methylated cytosines (G in the reverse sequencing marked by arrows) at the represented CpG sites, whereas all CpG cytosines are unmethylated in DNA from non-neoplastic brain tissue (A in the reverse sequencing marked by arrowheads).