Figure 1.

Confocal fluorescence images of MCF-7 cells double stained with Foscan® and organelle probes. Lysosomes were stained by overnight incubation with 125 μg ml⁻¹ LY, washed and incubated for 3 h with 1 μg ml⁻¹ Foscan® (row A). For other organelle probes, cells were sensitised with Foscan® (1 μg ml⁻¹, 3 h), washed and then subjected to organelle staining. Mitochondria were labeled with 5 μM Rh123 for 30 min (row B), Golgi apparatus was stained with 4 μM BPC (15 min) (row C) and ER with 2 μg ml⁻¹ DiOC₆ for 15 min (row D). Objective magnification×63.