Figure 3.

Intracellular fluorescence spectra of Rh123 alone (A). Intracellular fluorescence spectra (B) and fluorescence topographic profiles (C) of MCF-7 cells incubated for 3 h with 1 μg ml⁻¹ Foscan® and then with 10 μM Rh123 for 45 min. The spectrum in (B) was recorded at position 13.5 μm on the X-axis of the profile in (C). Fluorescence topographic profiles in (C) were recorded at 545 nm (Rh123) and at 654 nm (Foscan®). Excitation wavelength 435 nm, objective magnification×63, exposure time 4 s.