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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: J Neuroimmunol. 2005 Oct 17;170(1-2):93–104. doi: 10.1016/j.jneuroim.2005.09.003

Fig. 1.

Fig. 1

Both S. aureus and PGN augment CD14 protein expression in primary microglia. CD14 KO and WT microglia were stimulated with either 107 heat-inactivated S. aureus, 10 μg/ml PGN, or 100 ng/ml LPS for 24 h, whereupon whole cell extracts (40 μg of protein per sample) were prepared and analyzed for CD14 expression by Western blotting as described in the Materials and methods section. Duplicative blots were probed with an antibody specific for β-actin to verify uniformity in gel loading. Results are representative of three independent experiments.