Skip to main content
. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: J Neuroimmunol. 2005 Oct 17;170(1-2):93–104. doi: 10.1016/j.jneuroim.2005.09.003

Fig. 6.

Fig. 6

Expression of the secreted PRR pentraxin-3 is regulated by CD14-dependent signals in PGN-stimulated microglia. CD14 WT (A) or CD14 KO and WT (B) microglia were seeded at 2 × 106 cells per well in 6-well plates and incubated overnight. The following day, cells were stimulated with either 107 heat-inactivated S. aureus or 10 μg/ml PGN (A) or PGN only (B), whereupon total RNA was isolated and examined for PTX3 expression by qRT-PCR as described in the Materials and methods section. Each real-time PCR reaction was performed in duplicate for PTX3 and the ‘‘housekeeping’’ gene GAPDH. The level of gene expression was calculated after normalizing PTX3 signals against GAPDH and is presented in relative mRNA expression units (mean ± S.D. of fold-changes pooled from three independent experiments). Significant differences between CD14 WT untreated versus S. aureus- or PGN-stimulated microglia (A) and CD14 WT versus CD14 KO PGN-stimulated microglia (B) are denoted with asterisks (*p <0.05).