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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: Biol Chem. 2007 Dec;388(12):1301–1312. doi: 10.1515/BC.2007.149

Figure 6.

Figure 6

Effect of zinc deficiency on ZIP5 protein abundance in the pancreas and visceral yolk sac (VYS).

(A) Western blot detection of ZIP5 (top panels), ZIP4 (middle panel) and ZIP1 (bottom panel) in membrane proteins prepared from pancreas (top left) and VYS (top right) from pregnant dams fed a zinc-adequate (ZnA) or zinc-deficient (ZnD) diet for 6 days beginning on d8 of pregnancy. Note that ZIP4 and ZIP1 are not expressed in pancreatic acinar cells. (B) Immunoprecipitation and Western blot detection of ZIP5 in VYS and HEK 293 cells transfected with empty vector (−ve) or with (+ve) a ZIP5 expression vector. VYS sac and HEK 293 cells were sonicated in IP buffer and samples were precipitated overnight using normal rabbit IgG (nrIgG) or ZIP5 antiserum (αZIP5). Immune complexes were subjected to Western blot analysis using ZIP5 antiserum.