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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Am J Physiol Regul Integr Comp Physiol. 2008 Mar 19;294(5):R1474–R1481. doi: 10.1152/ajpregu.00130.2008

Figure 1. Confirmation of the genotype of triple-knockout mice with targeted deletions of the Zip1, Zip2 and Zip3 genes. Part A.

Figure 1

PCR genotyping of wild-type (WT) and knockout alleles for Zip1, Zip2 and Zip3 genes in triple-knockout (TKO) mice. PCR reactions contained three primers each that directed the amplification of both wild-type and knockout alleles of these Zip genes as described in Methods. Details of the targeting of each of theses genes have been published previously (5; 6; 33). M: DNA ladder. Part B. RT-PCR detection of Zip1, Zip2 and Zip3 mRNAs in total liver RNA from wild-type and triple-knockout mice. GAPDH served as an internal control for the RT-PCR, and n.c. indicates samples in which the reverse transcriptase was omitted from the reaction.