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. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2008 Jan 11;44(7):1420–1432. doi: 10.1016/j.freeradbiomed.2007.12.038

Figure 2.

Figure 2

Spectral deconvolution of Hb species absortption. (A) Typical fit of an absorption spectrum showing both the raw data and the fit. HbNO found was 29%, the percentage of oxyHb was 5%, deoxyHb was 21%, the percentage of metHb was 41%, and metHb-NO2− was 4%. The data were taken from a partially oxygenated Hb sample (100 μM, 36% oxygen saturated) after incubating with 100 μM AS for 33 minutes. (B) The basis spectra used for fitting.