Skip to main content
. Author manuscript; available in PMC: 2009 Apr 1.
Published in final edited form as: Free Radic Biol Med. 2008 Jan 11;44(7):1420–1432. doi: 10.1016/j.freeradbiomed.2007.12.038

Figure 4.

Figure 4

The reaction of Angeli’s salt with equi-molar oxyHb. (A) OxyHb (100 μM) was mixed with 100 μM Angeli’s salt in 0.1 M phosphate buffer under aerobic conditions. UV-Vis spectra were recorded at 3.0 min intervals after the initial scan. (B) Each spectrum from panel A was fit to basis spectra to determine the percentage of each species at each time point. (C) OxyHb (50 μM) was mixed with 50 μM Angeli’s salt in 0.1 M phosphate buffer under aerobic conditions. UV-Vis spectra were recorded at 3.0 min intervals after the initial scan. Note that the pathlength of the cell used here was smaller than that used to collect the data shown in panel A (0.2 cm compared to 0.5 cm). (D) Each spectrum from panel C was fit to basis spectra to determine the percentage of each species at each time point.