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The reaction between partially oxygenated hemoglobin with Angeli’s salt. Partially oxygenated hemoglobin (100 μM ) was mixed with 100 μM Angeli’s salt in deoxygenated phosphate buffer. (A) Raw absorption spectra. (B) Each spectrum was fit to basis spectra to determine the percentage of each species at each time point. (C) The average amount of each species formed at 72 minutes from three different experiments. Standard deviations are also shown.
