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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: J Leukoc Biol. 2008 Feb 13;83(5):1277–1285. doi: 10.1189/jlb.1107751

Figure 5. Lack of accelerated neutrophilic differentiation in LRG-transfected 32Dwt18 cells in response to Epo treatment.

Figure 5

(A) Cells stably transfected with vector alone or mLRG cDNA were washed out of IL-3 and transferred to Epo containing media. At the indicated time points, aliquots of cells were cytospun onto slides and neutrophilic differentiation monitored by Wright-Giemsa staining. (B) Bar graphs indicate the fraction of the total cell population transfected with vector alone or LRG at various stages of neutrophilc differentiation at each time point. (C) Aliquots of cells were stained with FITC-conjugated CD11b antibody and analyzed for CD11b expression by FACS analysis.