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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: Circulation. 2007 Jun 18;116(1):17–24. doi: 10.1161/CIRCULATIONAHA.107.708818

Figure 4.

Figure 4

Effect of suppression of Upf1 by RNAi on NMD of the R1014X mutation. HEK293 cells stably expressing the WT and R1014X minigenes were transfected with pSUPERpuro-hUpf1/I and pSUPERpuro-hUpf1/II (Upf1) or pSUPERpuro-scrambled (CON) constructs. A: Western blot analysis of Upf1 protein. B: Analysis of mRNA by RPA. The quantitative data after normalization using protected hygromycin resistant gene mRNA are plotted as percentage of WT control from four independent experiments. P-values are Bonferroni-corrected.