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. Author manuscript; available in PMC: 2008 May 12.
Published in final edited form as: Circulation. 2007 Jun 18;116(1):17–24. doi: 10.1161/CIRCULATIONAHA.107.708818

Figure 5.

Figure 5

Analysis of NMD in neonatal rat ventricular myocytes using R1014X minigene adenovirus constructs. Myocytes infected by WT and R1014X mutant minigene adenoviruses were treated (+) or not treated (-) with 100 μg/ml of CHX for three hours, and expressed mRNA analyzed by RPA. The mRNA from uninfected myocytes was used as a control (CON). The level of E2 transcripts (E2) from adenovirus served as a loading control. The quantitative data after normalization using protected E2 mRNA are plotted as percentage of WT control from four independent experiments. P-values are Bonferroni-corrected.