Table 1. Summary of caspase processing induced by cytochrome c in extracts from RCC cell lines.
| KTCTL-26A | KTCTL-30 | CAKI-1 | KTCTL-1M | KTCTL-84A | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cytochrome c (μg ml−1) | 1 | 50 | 1 | 50 | 1 | 50 | 1 | 50 | 1 | 50 |
| Caspase-3 | 0/1/1 | 2/1/2 | 1/1/2 | 2/2/2 | 1/1/1 | 2/1/2 | 1/1/1 | 2/2/2 | 1/1/1 | 1/2/1 |
| Caspase-9 | 1/2 | 3/3 | 3/3 | 3/3 | 2/3 | 3/3 | 3 | 3 | 2/2 | 3/3 |
| Caspase-7 | 1/3/3 | 3/3/3 | 3/3/3 | 3/3/3 | 3/3/3 | 3/3/3 | 3/3/3 | 3/3/3 | 2/3/1 | 3/3/3 |
Extracts from five RCC cell lines were incubated in the absence of cytochrome c or in the presence of 1 or 50 μg ml−1 cytochrome c for 1 h at 37°C. Reactions were then tested by immunoblotting for processing of caspase-3, -7 and -9. Semiquantitative values were allocated as follows: (0) no processing detectable; (1) processed caspase visible but no visible loss of procaspase; (2), decrease in the signal of the procaspase band visible; and (3), procaspase completely disappeared. Experiments were performed three times for caspase-3 and -7, one or two times for caspase-9. Each value represents one separate experiment.