Figure 3.

Radiation (6 Gy) improved the capability of A-172 and U-138 cells to attach to fibronectin (FN) and Matrigel after 48 h in comparison with BSA or polystyrene (P). To determine functional β1- and β3-integrin engagement in radiation-altered adhesion in these two cell lines, the cells were incubated with specific adhesion-blocking antibodies against β1- (mAb13) or β3-integrins (RUU-PL7F12) or with the disintegrin echistatin. Control experiments were performed using equivalent concentrations of unspecific anti-mouse IgG2a or IgG1 antibodies. Columns represent the mean ±s.d. of the optical densities (OD) at 630 nm representing cell adhesion to the different substrates of three independent experiments. Statistical analysis compared adhesion to FN or Matrigel of nonirradiated cells with adhesion to FN or Matrigel of irradiated cells. ^* P<0.01.