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. 2003 Nov 25;89(11):2122–2132. doi: 10.1038/sj.bjc.6601429

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Copyright © 2003 Cancer Research UK

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The in-vitro invasion assay indicated that irradiation (6 Gy) is able to inhibit strongly A-172 but not U-138, LN-229 and LN-18 cell invasion into Matrigel. To analyse the dependence of the invasion process on MMP-2 or β1- or β3-integrins, nonirradiated as well as irradiated cells were either incubated with the MMP-2/-9 inhibitor III (MI), anti-β1- (mAb13) and anti-β3-integrin (RUU-PL7F12) antibodies or the β1-/β3-integrin-blocking disintegrin echistatin alone or in combination. Additionally, control experiments were performed using equivalent concentrations of unspecific anti-mouse IgG2a or IgG1 antibodies. The data were collected by counting the number of cells per field (four high-powered fields). Columns represent the calculated mean ±s.d. of three independent experiments. Statistical significance was calculated by comparing the rate of invasion after anti-MMP and/or anti-integrin treatment to the rate of invasion of untreated cells. Furthermore, statistics were calculated by comparing the rate of invasion of treated cells after irradiation to the rate of invasion after irradiation alone. nt, no treatment; ^*P<0.01.